TCP Transcription Factors in Moso Bamboo (Phyllostachys edulis): Genome-Wide Identification and Expression Analysis.

Huan-Long Liu,Yan Xiang,Min Wu,Feng Chen,Ya-Meng Gao,Fei Li

doi:10.3389/fpls.2018.01263

Huan-Long Liu, Yan Xiang + Show 4 more

Open Access

https://doi.org/10.3389/fpls.2018.01263

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Abstract

TEOSINTE BRANCHED 1, CYCLOIDEA, and PROLIFERATING CELL FACTORS (T), members of a plant-specific gene family, play significant roles during plant growth and development, as well as in response to environmental stress. However, knowledge about this family in moso bamboo (Phyllostachys edulis) is limited. Therefore, in this study, the first genome-wide identification, classification, characterization, and expression pattern analysis of the TCP transcription factor family in moso bamboo was performed. Sixteen TCP members were identified from the moso bamboo genome using a BLASTP algorithm-based method and verified using the Pfam database. Based on a multiple-sequence alignment, the members were divided into two subfamilies, and members of the same family shared highly conserved motif structures. Subcellular localization and transactivation activity analyses of four selected genes revealed that they were nuclear localized and had self-activation activities. Additionally, the expression levels of several PeTCP members were significantly upregulated under abscisic acid, methyl jasmonate, and salicylic acid treatments, indicating that they play crucial plant hormone transduction roles in the processes of plant growth and development, as well as in responses to environmental stresses. Thus, the current study provides previously lacking information on the TCP family in moso bamboo and reveals the potential functions of this gene family in growth and development.

Highlights

Plants encounter a variety of environmental stresses during their growth and development; they possess various protective systems at the whole-plant, tissue, cellular, subcellular, genetic, and molecular levels (Sheshadri et al, 2016)
Prospective TCP members from the moso bamboo genome were obtained from BambooGDB using a BLASTP algorithm-based search with seed sequences from reported rice TCP proteins
The ABAresponsive elements (ABRE cis-acting element) (Shen and Ho, 1995) were found in the promoter regions of PeTCP6–8, 10, 12, 14, and 15. Among these TCP genes and environmental stressresponsive elements, we found that PeTCP9 and PeTCP14 had single types of responsive elements, salicylic acid (SA)-responsive elements and ABAREs, respectively

Summary

Introduction

Plants encounter a variety of environmental stresses during their growth and development; they possess various protective systems at the whole-plant, tissue, cellular, subcellular, genetic, and molecular levels (Sheshadri et al, 2016). Stress-inducible genes are the major molecular factors involved in environmental stress responses and increased tolerance (Yamaguchishinozaki and Shinozaki, 2005). These genes have generally been divided into two categories, one involved directly in stress tolerance and the other in signal transduction and transcriptional regulation. The TCP Gene Family in Moso Bamboo environment have been identified and reported in plants, such as MYB (salt and drought tolerance) (Zhang et al, 2012; Cui et al, 2013), ERF (ethylene responsive factors) (Lata et al, 2014), WRKY (regulation of stress response) (He et al, 2012; Yan et al, 2014; Cai et al, 2017), HD-Zip (drought and salt tolerance) (Zhao et al, 2011, 2014), and bZIP (pathogen defense regulation) (Sheshadri et al, 2016). A previous study reported that TCP TFs directly translate environmental signals to cope with environmental stresses during plant growth (Danisman, 2016)

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