Abstract
While best known for its role in the innate immune system, the TANK-binding kinase 1 (TBK1) is now known to play a role in modulating cellular growth and autophagy. One of the major ways that TBK1 accomplishes this task is by modulating the mechanistic Target of Rapamycin (mTOR), a master regulator that when activated promotes cell growth and inhibits autophagy. However, whether TBK1 promotes or inhibits mTOR activity is highly cell type and context dependent. To further understand the mechanism whereby TBK1 regulates mTOR, we tested the hypothesis that TBK1 phosphorylates a key component of the mTOR complex 1 (mTORC1), Raptor. Using kinase assays coupled with mass spectrometry, we mapped the position of the TBK1 dependent phosphorylation sites on Raptor in vitro. Among the sites identified in vitro, we found that TBK1 promotes Raptor Ser877 phosphorylation in cells both basally and in response to pathogen-associated molecules known to induce TBK1 activity. The levels of Raptor Ser877 phosphorylation were inversely correlated with the levels of mTOR activity. Expression of a mutant Raptor that could not be phosphorylated at Ser877 led to an increase in mTORC1 activity. We conclude that TBK1 limits mTORC1 activity by promoting Raptor Ser877 phosphorylation.
Highlights
TANK-binding kinase 1 (TBK1) is an IKK-related kinase that is known for its role in the innate immune system1
In a model of chronic immune activation induced by knockout of the Three-prime repair exonuclease 1 (Trex1), TBK1 was linked to suppression of mTOR complex 1 (mTORC1) activity11
In cell lines that were sensitive to TBK1 inhibition, TBK1 inhibitors decreased markers of mechanistic Target of Rapamycin (mTOR) activity, whereas the opposite occurred in TBK1 inhibitor resistant cell lines
Summary
TANK-binding kinase 1 (TBK1) is an IKK-related kinase that is known for its role in the innate immune system1. We found that TBK1 regulates Raptor Ser877 phosphorylation, both in cell-free kinase assays and in intact cells. The data presented indicates that Raptor Ser877 phosphorylation limits mTORC1 signaling and this may represent a mechanism whereby TBK1 limits mTORC1 activity.
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