Abstract
As one of the most common neoplasms globally, lung cancer (LC) is the leading cause of cancer-related mortality. Recurrence and metastasis negatively influencing therapeutic efficacy and overall survival demand new strategies in LC treatment. The advantages of TCM are increasingly highlighted. In this study, we obtained the major chemical components and their ratios in the aqueous extract of Taxus wallichiana var. chinensis (Pilg.) Florin (AETW) by UPLC-Q/TOF-MS/MS detection. The CCK-8 assay revealed that AETW could selectively inhibit the growth of A549 and HCC827 cells in a dose-dependent manner with little effect on normal human lung cells. Moreover, both in vitro and in vivo experiments showed that AETW was able to suppress the capacities of cell migration and invasion and downregulate the EMT and the JAK/STAT3 signaling pathway. To further probe into the molecular mechanism, the overexpression of STAT3 was performed into LC cells with AETW treatment, which counteracted the inhibitory effect on malignant behaviors of A549 and HCC827 cells with the decline in the expressions of p-JAK and p-STAT3. Taken together, we propose that AETW may inhibit the proliferation and metastasis by inactivating the JAK/STAT3 axis.
Highlights
Lung cancer (LC) remains a global public health problem characterized by its strong invasiveness and rapid metastasis (Ashrafizadeh et al, 2021a)
Taxus wallichiana var. chinensis (Pilg.) Florin was purchased from the pharmacy in the First Affiliated Hospital of Zhejiang Chinese Medical University (Hangzhou, China), which was manufactured by the Ningbo Taikang Yew Biological Engineering Company (Batch No 100513)
Our present study indicated that AETW might be a promising drug as a Janus kinase (JAK) activation inhibitor used in the targeted treatment of lung cancer (LC)
Summary
Lung cancer (LC) remains a global public health problem characterized by its strong invasiveness and rapid metastasis (Ashrafizadeh et al, 2021a). Despite improvements made in the diagnosis, surgical techniques, health management, and adjuvant therapy in recent years, LC is closely related to high mortality due to its malignancy and invasiveness (Ashrafizadeh et al, 2021b). Invasion and metastasis along with unlimited reproduction of LC cells induce poor response or even resistance to routine clinical strategies, further decreasing the survival (Abadi et al, 2021; Pacini et al, 2021). Searching for effective therapeutic strategies and targets is urgent and essential. It is worth mentioning that as an important source for the development of novel anti-tumor drugs, traditional Chinese medicine (TCM) is featured by the advantages of multiple targets, high efficacy, low toxicity, and low medical cost (Wang et al, 2021)
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