Taxus metabolomics: methyl jasmonate preferentially induces production of taxoids oxygenated at C-13 in Taxus x media cell cultures

Abstract

Cells from suspension cultures of Taxus cuspidata were extracted with pentane as a source of relatively non-polar taxoids. Of the 13 taxoids identified in this fraction, eight were oxygenated at C-14 and two had not been previously described. These taxoids, along with existing taxoid standards, were employed to profile the metabolites of Taxus x media cv . Hicksii cell suspension cultures induced with methyl jasmonate to produce paclitaxel (Taxol®). The majority of the taxoid metabolites produced in these induced cultures were oxygenated at C-13, and not C-14.