Abstract
A taxonomic methodology based on current keys and the ready availability of refrigerated slant media is presented. It utilizes defined media, surface inoculation, and reagent indicators which simplify final readings. Yeast Nitrogen Base w/o Amino Acids or Ammonium Sulfate (DIFCO) is used as a source of vitamins and trace nutrilites. The medium consists of the Seitz-filtered vitamin base (0.1 % w/v), nitrogen source (0.5 %), and carbon source (1.0 %) mixed into 1.5 % agar slants. Test inocula are taken from a vitamin base-ammonium sulfate-dextrose positive control slant. Cultures are incubated for 2–4 days at 25° C. Nitrate assimilation is tested with the usual sulfanilic acid-alphanaphthylamine reagents. Carbon assimilation is tested with 0.04 % aqueous brom-phenol-blue. These reagent tests help discriminate between negative, weak, and doubtful test results. Tables of test results onCryptococcus, Rhodotorula, and non-fermentingTorulopsis species are presented.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
