Abstract

Four strains of “wild”-type sporeforming, crystalliferous bacilli were isolated from dead or diseased gypsy-moth larvae. Morphological and physiological characteristics of these strains, together with the Mattes strain of Bacillus thuringiensis and an acrystalliferous strain, were studied. Results showed that the four crystalliferous bacilli conform to the description of B. thuringiensis var. thuringiensis Berliner ( de Barjac and Bonnefoi, 1962; Bonnefoi and de Barjac, 1963) and should belong to serotype 1-Berliner. All crystalliferous isolates were found to be pathogenic. In feeding trials employing 3,900 instar II larvae, significant differences were observed in the toxicity of the laboratory-prepared crystal-spore powders of each “wild” strain.

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