Abstract
The identification of yeasts or yeasts-like fungi and verify their diversity are principal aspect for bioindustry and ecosystem sustainability. Taxonomic approach provides identification tool to ensure the taxonomic position of yeasts and yeasts-like fungi which definitely set to utilization concerns. The aim of this study is to understanding the taxonomic position of yeasts and yeasts-like fungi from the distinctive of its sequences relationship. Yeasts and yeasts-like fungi strains were isolated through various culture dependent methods from natural resources samples of Karimun Besar Island, Province of Riau Islands, Indonesia. The identification process was performed through amplifying the accurate DNA-based in D1/D2 region of large subunit (26S) ribosomal DNA. As the result, a total of 85 isolates of yeasts and yeasts-like fungi were obtained with 16 closest related taxa through phylogenetic tree construction. Ascomycetous was the predominating group representing 91% of the total isolates sequences followed by Basidiomycetous (8%) and Zygomycetous (1%). The black yeasts (yeasts-like) known as Aureobasidium melanogenum was predominant species with represent to 54% of total isolates and present in particular habitat. Taxonomically, there are six isolates are represent to be novel taxa candidates which pretend to enhance genetic resources of yeasts and yeasts-like fungi especially from Indonesia. In addition, this information provides specific technique to reach specific yeasts or yeasts-like fungi species in nature by managing the sample collection and culture methods.
Highlights
Yeasts have been exploited for many decades by scientist
To understand the taxonomic position of the yeasts and yeasts-like fungi, we explore them by molecular approach to construct their diversity through the phylogenetic tree
Isolation method and preservation Isolation was conducted in Biosystematic of Yeast Laboratory-Indonesian Culture Collection (InaCC), Microbiology Division, Research Center for Biology, Indonesian Institute of Sciences (LIPI)
Summary
Yeasts have been exploited for many decades by scientist. They are important microbes for bioindustry and ecosystem services. Discovering biodiversity and elucidating the taxonomic position of yeasts and yeasts-like fungi are necessary for conservation, quality control, and ecological monitoring (Xu, 2016). Taxa position of yeasts are commonly using some criteria based on septal morphology, cell wall composition, chemical characteristic, and conserve genes analysis (Fell et al, 2000; Kurtzman et al, 2011). Identification of yeasts was only used conventional method based on morphological and chemical analyses Those methods took many time and expensive (Fell et al, 2000; Xu, 2016). Based on conserve genes database, we are able to mapping the taxonomical position of our isolates. The diversity of yeasts and yeasts-like fungi are necessary for industry, ecosystem management and genetic resources database from Indonesian natural resources
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