Abstract
Taxol Production by Fusarium Arthrosporioides Isolated from Yew, Taxus CuspidataA taxol-producing endophyte wasFusarium arthrosporioides.As a novel taxol resource, a taxol-producing endophyte was successfully isolated from the yew tree,Taxus cuspidataSieb. et Zucc. According to the morphological characterization and the ITS4-ITS5 sequences, the isolated endophytic fungus was identified asFusarium arthrosporioides.Fermentation conditions for taxol production were optimized with the isolated strain (F-40) ofF. arthrosporioides.The fungal taxol was analytically confirmed by TLC, RP-HPLC, LC-MS and NMR.F. arthrosporioidesisolated from yew was found to produce taxol with a maximum yield of 131 μg/L. Precise methods were established for detecting the fungal taxol and its derivatives.
Highlights
The resource of Taxus trees worldwide is very limited [1], and recovery of taxol from the tree bark generally means fatal damage to the trees
As a novel taxol resource, a taxol-producing endophyte was successfully isolated from the yew tree, Taxus cuspidata Sieb. et Zucc
According to the morphological characterization and the ITS4-ITS5 sequences, the isolated endophytic fungus was identified as Fusarium arthrosporioides
Summary
The resource of Taxus trees worldwide is very limited [1], and recovery of taxol from the tree bark generally means fatal damage to the trees. Taxol content in the bark is quite low (< 0.02% by weight). Finding other sources of taxol has been a research topic. Since 1993, several endophytic fungi, such as Taxomyces andreanae and Pestalotiopsis microspore, etc., have been reported to produce taxol [2, 3]. Produce taxol, but only at low levels, 24–1081 ng/L [2,3,4,5]. We report a taxol-producing Fusarium arthrosporioides with a yield of 131 μg/L
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