Taurine efflux, band 3, and erythrocyte volume of the hagfish (Myxine glutinosa) and lamprey (Petromyzon marinus)

Abstract

AbstractHypoosmotic stress in skate RBC causes cell swelling followed by the release of solutes (osmolytes) to bring about a regulatory volume decrease (RVD). The β‐amino acid, taurine, is a primary osmolyte in RVD of skate RBC. Taurine efflux is inhibited by stilbene disulfonates and other inhibitors of the anion exchanger, band 3, suggesting that band 3 is involved in the release of taurine during RVD in skate RBC. Since RBC of the cyclostomes, hagfish and lamprey, are reported to be deficient in band 3, we examined taurine efflux, quantity of band 3, and volume response in these RBC. There was no significant increase in taurine efflux when hagfish RBC were exposed to hypoosmotic medium, and the rate of taurine efflux in these RBC was markedly lower than that in skate RBC. Hagfish RBC plasma membranes show only 11% of the level of 3H2DIDS bound to skate RBC membranes. In addition, DIDS‐sensitive sulfate exchange is much lower in hagfish compared with skate RBC. Similarly, lamprey RBC showed little taurine efflux compared with skate, and the amount of 3H2DIDS binding to these RBC membranes was 24% that of skate. Finally, although both hagfish and lamprey RBC swell in hypoosmotic medium, the lamprey cells show an RVD while the hagfish cells do not. Thus, in the RBC of the two cyclostomes, one showing an RVD and the other not, low band 3 is associated with low volume‐activated taurine efflux. These results support the hypothesis that band 3 is involved in volume‐activated taurine efflux. © 1992 Wiley‐Liss, Inc.