Abstract
A hypercholesterolemia diet has been associated with the hepatic and cardiac abnormalities and the pathological changes. The present work was designed to investigate the histological and immunohistochemical changes in the liver and heart of rabbits when fed high fat diet and the possible protective role of an antioxidant "taurine". Twenty-four male white New Zealand rabbits were divided into four groups, 6 rabbits each. Group 1, served as a control, rabbits fed with a normal diet. Group 2, (taurine group), rabbits were given orally taurine (10 mg Kg-1 b.w/day) for 8 weeks. Group 3 (hypercholesterolemic group), rabbits fed (2% cholesterol-enriched diet for 8 weeks. Group 4, rabbits fed 2% cholesterol-enriched diet plus taurine (10 mg Kg-1 b.w/day) orally for the same period. Histopathological examinations revealed that high cholesterol diet caused hepatic and myocardial tissue changes compared with rabbits fed with a normal diet. Including fatty degeneration, inflammations and necrosis of Hepatocytes and vacuolar degeneration, disorganization of myofibrils and necrosis of myocardial cells. Immunohistochemistry for caspase-3 for apoptosis were performed. Caspase-3 positive cells in the liver tissue and Caspase-3 positive area in myocardial tissue increased in high cholesterol diet group. Taurine markedly attenuate hypercholesterolemia-induced cardiac and hepatic histopathological changes in the cholesterol plus taurine group compared to the cholesterol group. Thus, the results suggest that taurine could play a beneficial role against hypercholesterolemia-induced complications in the liver and heart of the rabbits.
Highlights
High-cholesterol dietary is known to play an oxidative stress in various organs such as the liver, heart important role in the induction of chronic diseases such and kidney (Du et al, 2010)
The histological examination of rabbit liver sections revealed that control and taurine groups had a normal architecture with radially arranged hepatocytes around to centrilobular vein (Fig. 1A,B)
The present results revealed that rabbits fed high cholesterol diet leads to several abnormalities in liver sections such as degree of fat accumulation, inflammatory infiltration and necrosis of hepatocytes
Summary
High-cholesterol dietary is known to play an oxidative stress in various organs such as the liver, heart important role in the induction of chronic diseases such and kidney (Du et al, 2010). Hypercholesterolemia as cardiovascular disease and hepatic abnormalities results in free radical production and thereby elevates especially in developing countries (Vinaixa et al, 2010). Several studies have demonstrated that dietary taurine showed a notable cholesterollowering effect in hypercholesterolemic rats fed a high cholesterol diet (Masuda and Horisaki, 1986; Murakami et al, 1999; Park and Lee, 1998; Hagar, 2004). Membrane stabilizing effect, regulates intracellular Ca2+ concentration, inhibits apoptosis, reduces the levels of pro-inflammatory cytokines in various organs and controls blood pressure (Das et al, 2008; 2010; 2011; Manna et al, 2008; 2009). The present study aimed to investigate the protective role of taurine against liver and heart tissues of rabbits fed a hypercholesterolemic diet
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
