Taurine Attenuates Carcinogenicity in Ulcerative Colitis-Colorectal Cancer Mouse Model.

Guifeng Wang,Hatasu Kobayashi,Shosuke Kawanishi,Mariko Murata,Ning Ma,Feng He,Shinji Oikawa

doi:10.1155/2020/7935917

Abstract

Taurine (2-aminoethane-sulfonic acid) is a type of amino acids and has numerous physiological and therapeutic functions, including anti-inflammation. However, there are few studies on the anticancer action of taurine. Our previous studies have demonstrated that taurine exhibits an apoptosis-inducing effect on human nasopharyngeal carcinoma cells in vitro. In this study, we have investigated whether taurine has an anticancer effect, using azoxymethane (AOM)/sulfate sodium (DSS)- induced mouse model for colon carcinogenesis. All mice, except those in control group, received a single intraperitoneal injection of AOM and DSS in the drinking water for 7 days twice, with 1-week interval. After the first DSS treatment, mice were given distilled water (model group) or taurine in the drinking water (taurine group) ad libitum. No tumor was observed in the control group. Taurine significantly suppressed AOM+DSS-induced tumor formation. Histopathological examination revealed AOM/DSS treatment induced colon cancer in all mice (8/8, 100%), and taurine significantly inhibited the progression of colon cancer (4/9, 44.4%). Taurine significantly attenuated cell proliferation in cancer tissues detected by Ki-67 staining. Taurine significantly increased the levels of an apoptosis marker cleaved caspase-9 and tumor suppressor protein PTEN. This is the first study that demonstrated that taurine significantly reduced carcinogenicity in vivo using AOM/DSS-induced colon cancer mouse model.

Highlights

Taurine (2-aminoethane-sulfonic acid) is a special amino acid containing sulfonate group and lacking carboxyl group and is found in high concentrations in many cells
After the first DSS treatment, the mice were randomly divided into two groups (n = 9, each) for DW and 0.5% (W/V) taurine in drinking water, ad libitum
The AOM+DSS mouse model was induced by intraperitoneal injection of AOM followed by two cycles of DSS exposure (Figure 1)

Summary

Introduction

Taurine (2-aminoethane-sulfonic acid) is a special amino acid containing sulfonate group and lacking carboxyl group and is found in high concentrations in many cells. Humans can endogenously synthesize taurine, but primarily depend on their diet for taurine, mostly found in seafood [1]. It is considered a conditionally essential nutrient. Taurine has different biological effects in various systems or organs, such as the cardiovascular system, skeletal muscle, retina, liver, kidney, and nervous system [3, 5, 6]. Many useful effects of taurine intake are reported, there are few studies about the anticancer action of taurine. Our previous studies have demonstrated that taurine exhibits an apoptosis-inducing effect on human nasopharyngeal carcinoma cells in vitro [10, 11]. Suzuki et al [12] demonstrated

Methods

Results

Conclusion