Abstract
Glutathione S-transferases (GSTs) have medically and agriculturally important roles in plants such as herbicide detoxification, responses to biotic and abiotic stress and catalytic reaction of anticancer compounds. The fully sequenced Arabidopsis thaliana and rice (Oryza sativa) genome revealed identification of 52 and 61 members of GSTs, and 28 and 39 of those belong to the Tau class of GSTs (GSTUs), respectively. Based on the sequences of AtGSTUs, 14 BAC clones derived from Brassica rapa that contain similar sequences of AtGSTUs were identified and 17 unique sequences of open reading frames were detected in the 14 BAC clones using blast search and sequence alignment. Those sequences were designated the Tau class candidate of GST derived from B. rapa (BrGSTUs) and it is confirmed that BrGSTUs are also clustered in tandem as it has been known to be common feature in plant. They were mapped on BrR5, BrR7, BrR8, BrR9, or BrR10, and their nucleotide and amino acid sequences were highly similar to those of AtGSTUs. In addition, in silico analysis of BrGSTUs was performed using Korea Brassica Genome Project 24 K (KBGP-24K) oligochip and microarray database for cold, salt, and drought stresses. The results of this study will facilitate further researches for breeding of Chinese cabbage containing medically and agriculturally important traits.
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