Taste reward modulates neuronal responses to preceding visual stimuli in the rat V2 neocortex

Abstract

Event Abstract Back to Event Taste reward modulates neuronal responses to preceding visual stimuli in the rat V2 neocortex Istvan Hernadi1*, Norbert Burkus1, Gergely Orsi1, Anita Kovacs1 and Lorand Kellenyi1 1 Department of Experimental Zoology and Neurobiology, Institute of Biology, University of Pecs, Hungary With a newly developed technique, a universal biopotentiostat and biological amplifier were combined for neurophysiological and electroanalytical measurement purposes to detect rapid changes in extracellular neurotransmitter levels, whilst recording in vivo single unit activity of the target neurons. Using carbon-fibre multibarrel electrodes, and microiontophoresis pumps in the same experiment, enabled neurochemical identification of the target cells. To test the validity of the above technique under in vivo conditions, we recorded neuronal activity (n=185) in the visual neocortex (V2ML) of 38 anesthetized wistar rats. During recording, we performed standard Pavlovian reward conditioning, using two types of light flashes (high power LEDs) as conditioning stimuli (CS+) or yoked randomised light flashes (CS-) as control stimuli. In each trial, the CS+ was present for 1000ms, followed by 500ms delay. We applied ~0,35ml 0,5M sucrose solution as unconditioned stimulus (US). At the end of each trial, distilled water was applied to the mouth. Twenty-five per cent of all neurons showed response to CS+ or CS- outside the context. Among light responsive neurons, a majority of 34 % developed a context-dependent (CS-US) response, consequently taking part in the rapid, light-associated learning procedure. Light responses of neurons changed only to previously paired stimulus (CS+), but remained constant to the yoked light stimulus (CS-). We successfully used the combination of neurophysiological and electrochemical techniques to describe rapid stimulus-reinforcement learning in the rat V2 under in vivo conditions. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Research on the cerebral cortex and related structures Citation: Hernadi I, Burkus N, Orsi G, Kovacs A and Kellenyi L (2009). Taste reward modulates neuronal responses to preceding visual stimuli in the rat V2 neocortex. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.196 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 06 Mar 2009; Published Online: 06 Mar 2009. * Correspondence: Istvan Hernadi, Department of Experimental Zoology and Neurobiology, Institute of Biology, University of Pecs, Pecs, Hungary, hernadi@gamma.ttk.pte.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Istvan Hernadi Norbert Burkus Gergely Orsi Anita Kovacs Lorand Kellenyi Google Istvan Hernadi Norbert Burkus Gergely Orsi Anita Kovacs Lorand Kellenyi Google Scholar Istvan Hernadi Norbert Burkus Gergely Orsi Anita Kovacs Lorand Kellenyi PubMed Istvan Hernadi Norbert Burkus Gergely Orsi Anita Kovacs Lorand Kellenyi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.