Abstract
To formulate and evaluate polymeric liposomes (PL) nanoparticles as a novel non-viral gene delivery. To explore its applicability and feasibility as a non-viral vector for gene transportation. Experimental study. To construct the orongoxygen induced retinopathy (OIR) mouse (C57BL/6J) model on the basis of improved Smith's methods. Western blot was used to measure VEGF protein expression in retinal tissue at P17 and P22. HE staining and fluorescein-dextran angiography of retinal vascular were performed to observe the morphologic alterations of retinal neovascularization. Frozen-section was used to show the membrane translocation of PL. In fluorescence angiograms, irregular neovascularization and fluoresce leakage were observed in OIR model. The results of Western blot showed that VEGF protein in retinal tissues were significantly different among groups (F = 158.207, P = 0.000) at P17 and P22 (F = 25.695, P = 0.000). The protein level was lower in both PL (0.70 ± 0.03) and Lipo group (0.66 ± 0.04) at P17 (P = 0.092), and the lower level was presented at P22 in PL group (0.50 ± 0.03) than in Lipo group (0.53 ± 0.05) (P < 0.05). HE staining were performed to observe the significantly improvements of retinal neovascularization in PL (28.0 ± 3.44) and Lipo group (24.50 ± 3.06) at P17. Moreover, inhibitory effects maintained at P22 in PL group (11.70 ± 3.09) as HE staining showed. Fluorescein angiography of retinal vascular showed retinal non-perfusion and neovascularization areas were smaller in both PL and Lipo group at P17. Frozen-section examination showed the property of membrane translocation. GFP expression could be seen in vitreous cavity just at first day post-intravitreal administration in PL and Lipo group, which could reach their peaks in external retina nearby RPE layer at P17, remaining at P22 in PL group. The PL performed excellent ability of membrane translocation and it was a kind of slow steady released gene vector.
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