Targeting of FliC gene for the identification of Salmonella Typhimurium in Poultry chicken by PCR in District Swat

Abstract

Salmonella is one of the prominent causes of food borne infection all over the world. The present study was performed to examine the existence of Salmonella typhimurium in poultry chicken samples and chicken wooden cutting boards. For this purpose, a total of 100 samples were collected to detect S. typhimurium. The samples were streaked on Salmonella Shigella agar media (SS media) for the growth of Salmonella. Out of total samples, 50% were appeared as positive on media plates. Total percentage of Samonella by sample wise was found to be higher in the liver (85%) followed by chicken wooden cutting boards (63%), food and proventriculus (50%), oesophagus and tissues (33%). Total percentage of Salmonella by area wise was found to be higher in Charbagh and Koza Bandai and Kanju (80%), Khwazakhela (55%), Mingora (55%), Sambat and Matta (30%). DNA was isolated from the positive samples and amplified with FliC gene-specific primer using optimized PCR protocol for the confirmation of S. typhimurium. In the screened samples, 28% of S. typhimurium were observed as positive samples by amplifying FliC gene sequence having a molecular band size of 620bp. Out of the total S. typhimurium positive samples, 6 were positive from Khwazakhela, 2 from Charbagh, 4 from Sambat and Matta and 2 from Mingora.