Abstract
The Schiff base (H2SALNN) (N,N′-bis(4-methoxy-salicylaldehyde)azine) has been designed to develop a DNA targeted fluorescent probe. The H2SALNN was synthesized and geometry optimized by DFT/B3LYP. The interaction of H2SALNN with Calf thymus DNA (CT-DNA) was studied by spectroscopic and calorimetric techniques. The compound was found to bind with CT-DNA through groove binding mode. From isothermal calorimetry (ITC) titration experiment, the binding constant between the compound and DNA was estimated to be (1.52 ± 0.03) × 105 M−1. The negative ΔG0 and positive ΔS0 values obtained from the calorimetric study confirmed the spontaneity of the binding of H2SALNN with DNA. Analysis of thermodynamic parameters indicated that the process of interaction of H2SALNN with DNA is entropy driven.
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