Targeting Mice Mammary Adenocarcenoma Cells with Zinc Oxide Nanoparticles

Abstract

The cytotoxic effect of zinc oxide nanoparticles against mice mammary adenocarcenoma cells wascarried out in vitro. The used nanoparticles were synthesized by pulsed laser ablation in liquidmethods; the used liquid was deionized water. Colloidal suspension of zinc oxide nanoparticles wassynthesized by Q-switched Nd: YAG pulse laser device (1064 nm) laser energy was 600 mJ and pulseduration was 10 nanosecond. High purity metallic zinc plate was submerged in 10 ml deionizedwater in 50 ml glass baker and ablated with 1000 pulse at room temperature. The nanospecificitiesof the synthesized nanoparticle colloidal were characterized with UV-Vis scanningspectrophotometer. Atomic force microscope was used to determine the particle size distribution andparticles shape. The ZnO crestless formation was characterized by X-ray diffraction.Mice mammary adenocarcenoma cells was treated with 25, 50, 100 ppm of ZnO nanoparticles.Results indicated a significant toxicity of the ZnO nanoparticles toward cancer cells, this toxicitycorrelated directly with ZnO nanoparticles elevated concentrations. These result need to be furtherinvestigated and the molecular mechanism of ZnO nanoparticles effect should be more clarify.