Targeting a Plasmodium vivax merozoite surface protein 1 fragment to the DEC205+ dendritic cell population elicits strong antibody and T cell responses.

Abstract

Event Abstract Back to Event Targeting a Plasmodium vivax merozoite surface protein 1 fragment to the DEC205+ dendritic cell population elicits strong antibody and T cell responses. Kelly N. Amorim1, Marcio M. Yamamoto1 and Silvia B. Boscardin1* 1 University of Sao Paulo, Brazil Dendritic cells (DCs) are critical in the interaction between the innate and adaptive immune systems, as they are able to process and present antigens to T and B cells. In the last decade, studies from several groups have shown that it is possible to target antigens directly to different DC populations using monoclonal antibodies (mAbs) to receptors present on the DC surface fused with the antigen of interest. An anti-DEC205 mAb has been used successfully to target antigens to the DEC205+CD8α+ DC population. The administration of low doses of the fusion mAb together with DC maturation stimuli is able to activate antigen-specific T cells and induce production of high antibody titers. Here we genetically fused the αDEC205 mAb with the 42kDa fragment derived from the Plasmodium vivax merozoite surface protein 1 (MSP1), a candidate vaccine for malaria. During Plasmodium invasion into the red blood cell, the 42kDa fragment is further cleaved into 33 and 19 kDa fragments. The 19kDa fragment is normally target for antibody response while the T cell epitopes are restricted to the 33kDa portion of the molecule. The administration of two doses of the αDEC-MSP1(42) fusion mAb in the presence of the TLR3 agonist poly I:C to either C57BL/6 or C57B10.A mice induced high anti-MSP1(19) antibody titers in the immunized mice. We also detected strong T cell immunity against a peptide present in the MSP1(33) sequence. Overall, our results indicate that targeting a Plasmodium vivax antigen to the DEC205+CD8α+ DC population improves B and T cell responses. Acknowledgements This work was supported by the Brazilian National Research Council (CNPq)/National Institutes of Science and Technology in Vaccines (INCTV, 15203*12), Sao Paulo State Research Funding Agency (FAPESP, 2007/08648-9) and by the BNP-Paribas Bank. K.N.S. Amorim received a fellowship from CNPq. Keywords: antigen targeting, Dendritic Cells, Merozoite Surface Protein 1, antibody response, T cell responses Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Amorim KN, Yamamoto MM and Boscardin SB (2013). Targeting a Plasmodium vivax merozoite surface protein 1 fragment to the DEC205+ dendritic cell population elicits strong antibody and T cell responses.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00557 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 17 May 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Silvia B Boscardin, University of Sao Paulo, Sao Paulo, Brazil, sbboscardin@usp.br Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Kelly N Amorim Marcio M Yamamoto Silvia B Boscardin Google Kelly N Amorim Marcio M Yamamoto Silvia B Boscardin Google Scholar Kelly N Amorim Marcio M Yamamoto Silvia B Boscardin PubMed Kelly N Amorim Marcio M Yamamoto Silvia B Boscardin Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.