Targeted multifunctional nanoparticles probe mediated photoacoustic imaging and treatment study of breast carcinoma in vitro

Abstract

Objective To investigate the ability of targeting in vitro breast carcinoma and photoacoustic-mediate apoptosis of breast carcinoma of the nanoparticle probe loaded with Fe3O4 and observe its consequence of photoacoustic imaging in vitro. Methods The polymeric multifunctional nanoparticles probe that was loaded with Fe3O4 and connected with Herceptin targeting breast carcinoma was prepared in the use of double emulsion method and carbodiimide method. General physical property, the condition of Herceptin connected with nanoparticles and the ability of targeting of the probe were tested. The different concentration of nanoparticles was imaged by photoacoustic. The inhibiting effect of targeting nanoparticles on breast carcinoma cells was evaluated in vitro. Results The targeting polymeric multifunctional nanoparticles probes loaded with Fe3O4 were prepared successfully with average particle diameter of (235.4±53.75)nm and Zeta potential (-13.4±4.7)mV. Fe3O4 particles dispersed on the shell of the probes. Antibody Herceptin was successfully connected with the surface of the nanoprobes. There were massive targeting nanoprobes surround the breast carcinoma cell strains SKBR3 in the targeting group in vitro. The photoascoustic signal of the nanoprobes enhanced with the increase of Fe3O4 concentration in photoacoustic experiment in vitro. The apoptotic rate of breast carcinoma increased after the laser irradiation in the cell inhibition experiment in vitro. That proved the obvious inhibition of breast carcinoma cells was caused by photothermal effect of the prepared nanoprobes. Conclusions The prepared polymeric multifunctional nanoparticles probe that was loaded with Fe3O4 and connected with Herceptin targeting breast carcinoma can be used as a photoacoustic contrast agent, which can inhibit the proliferation of breast carcinoma by targeting photoacoustic therapy. Key words: Photoacoustic techniques; Targeted nanoparticle probe; Breast neoplasms; Magnetite nanoparticles