Abstract
LC-MS/MS-based molecular networking facilitated the targeted isolation of a new cyclic hexadepsipeptide, cymodepsipeptide (1), and two known analogues, RF–2691A (2) and RF–2691B (3), from the fungus Cymostachys sp. NBUF082 that was derived from a mesophotic zone Aaptos sponge collected near Apo Island. The constitution and configuration of 1 was elucidated through 1D and 2D NMR-spectroscopy, high resolution mass-spectrometry, and chemical degradations including Marfey’s analysis and chiral HPLC. It was observed that 1 was moderately cytotoxic against CCRF-CEM human acute lymphocytic leukemia cells in vitro with the IC50 value of 9.2 ± 1.1 μM.
Highlights
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NBUF082 fungus associated with a mesophotic zone Aaptos sp. sponge, after re-mining
The molecular networking cluster that contained the compounds targeted indicated more cyclic hexadepsipeptides in addition to 1–3, which may have been below isolable quantities but could remain to be discovered in a scaled-up sample production and regulation of peptide gene expression by nutrients and genetic engineering technology
Summary
Academic Editors: Bin Wu and Ikuro Abe. Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Especially sponge-derived microorganisms, has afforded a prolific library of new and interesting natural products for drug discovery [1,2,3,4]. From such marine biodiversity, the global marine pharmaceuticals pipeline website
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