Targeted insertion of Cre recombinase into the TNAP gene: Excision in primordial germ cells

Abstract

system are a powerful approach to study thefunction of genes in particular cell lineages and forwhich generalized null mutations result in early lethalphenotypes (Lobe and Nagy, 1998). Here we report thecreation of a primarily PGC (primordial germ cell) spe-cific Cre recombinase transgenic line, designated TNAP-Cre. In this strain, the Cre recombinase has beenknocked into the locus of the TNAP (Tissue Non-SpecificAlkaline Phosphatase) gene.The structure of the TNAP-Cre targeting construct isdepicted in Figure 1a. After electroporation into R1 ES(Nagy