Abstract
BackgroundPostnatal olfactory bulb (OB) neurogenesis involves the generation of granule and periglomerular cells by neural stem cells (NSCs) located in the walls of the lateral ventricle (LV). Recent studies show that NSCs located in different regions of the LV give rise to different types of OB neurons. However, the molecular mechanisms governing neuronal specification remain largely unknown and new methods to approach these questions are needed.ResultsIn this study, we refine electroporation of the postnatal forebrain as a technique to perform precise and accurate delivery of transgenes to NSCs located in distinct walls of the LV in the mouse. Using this method, we confirm and expand previous studies showing that NSCs in distinct walls of the LV produce neurons that invade different layers of the OB. Fate mapping of the progeny of radial glial cells located in these distinct LV walls reveals their specification into defined subtypes of granule and periglomerular neurons.ConclusionsOur results provide a baseline with which future studies aiming at investigating the role of factors in postnatal forebrain neuronal specification can be compared. Targeted electroporation of defined LV NSC populations will prove valuable to study the genetic factors involved in forebrain neuronal specification.
Highlights
Postnatal olfactory bulb (OB) neurogenesis involves the generation of granule and periglomerular cells by neural stem cells (NSCs) located in the walls of the lateral ventricle (LV)
We assessed the number of radial glial cell (RGC) expressing green fluorescent protein (GFP) 1 day after the electroporation of a GFP-encoding plasmid (Figure 1B); throughout the rostro-caudal extent of the LV
The position of GFP+ RGCs (Figure 1B) was superimposed on the drawings, and a line defining the extension of the electroporated area was added (Additional file 1)
Summary
Postnatal olfactory bulb (OB) neurogenesis involves the generation of granule and periglomerular cells by neural stem cells (NSCs) located in the walls of the lateral ventricle (LV). Recent studies have revealed continuity in the development of forebrain germinal zones, with many regions of the embryonic neuroepithelium (that is, the medial and lateral ganglionic eminence as well as the pallium) contributing to the adult SVZ [4]. In agreement with their distinct developmental origin, the lateral ventricle (LV) walls contain progenitor populations that are biased to acquire defined OB neuronal fates [4,5,6]. This early specification appears to reflect an intrinsic property of the cells, as defined by ectopic transplantation experiments [5,6]
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