Targeted and Conditional Gene Expression Workshop, 8th International Conference on Zebrafish Development and Genetics

Abstract

Several technologies for studying gene function in zebrafish embryos, larvae, and adults were discussed at the Targeted and Conditional Gene Expression workshop. The workshop included six oral presentations followed by a community discussion of current approaches, their limitations, and possible solutions. The research talks focused on new methods for controlling the activity of endogenous or exogenous genes through genetic and/or chemical strategies. Approaches for conditionally regulating endogenous gene function included short-hairpin RNA (shRNA) expression, reversible transposon-induced mutations, and caged morpholinos. Technologies for controlling exogenous gene activity included a chemically inducible progesterone receptor-based transactivator, the Cre-LoxP system, and estrogen receptor fusion proteins. General considerations for using the Gal4/UAS system in zebrafish were also discussed.