Abstract
A ratiometric fluorescent aptasensor based on energy transfer between [Ru(bpy)3]2+ and silica quantum dots (silica QDs) for assaying OTA was fabricated. Theaptamer for OTA was used as the gate to shield the fluorescent reagent [Ru(bpy)3]2+ into mesoporous silica nanoparticle (MSN). In the presence of OTA, the constrained [Ru(bpy)3]2+ was released from MSN due to atarget-induced aptamer conformational change. The released [Ru(bpy)3]2+ adsorbed onto the negatively charged silica QDs through electrostatic interaction. This createsappearance of fluorescence from [Ru(bpy)3]2+ at 625nm and decrease of the fluorescence from silica QDs at 442nm owing to the energy transfer. The value of FL625nm/FL442nm was in proportion to the concentrationof OTA in the range 0.5~100ngmL-1 with aLOD of 0.08ngmL-1. Practical applicability of this method was validated by thedetermination ofOTA in flour samples. Graphical abstract The sensing principle of this sensor.
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