Abstract
Laser-capture microdissection (LCM) followed by realrime reverse transcription polymerase chain reaction (TaqMan®) is an alternative approach for localizing gene targets in tissue sections when in situ hybridization (ISH) is problematic. LCMITaqMan® and nonisotopic ISH were used to qualitatively and quantitatively evaluate mRNA expression of neuromedin U receptor and Rho kinase in sections of human atherosclerotic plaque. LCM/TaqMan® successfully localized Rho kinase to specific tissue elements when ISH was unsuccessful. LCM/TaqMan® is a powerful alternative/adjunct to ISH because it provides quantitative expression data, has greater sensitivity for detecting low abundance transcripts, and is less sensitive to partial RNA degradation. (The J Histotechnol 28:177, 2005)Submitted November 22, 2004; accepted with revisions June 3, 2005
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