Abstract
Polycarbonate (PCL) has been widely used in tissue engineering, but its hydrophobicity and low biological activity limit its further promotion and application. By adding nanoparticles, the hydrophilicity and biological activity of PCL can be improved. In this study, different amounts of Ta (1–10%wt) were added to PCL, and then their mechanical and biological properties were studiedin vitro. XRD found that 5%Ta-PCL has the highest crystallinity. At the same time, cell experiments CCK8, cell adhesion, osteogenic differentiation, and osteogenesis related gene expression showed that Ta can enhance the mechanical and biological properties of PCL, while 5% Ta-PCL showed the best mechanical and biological properties. This composite of tantalum and PCL could have a clinical potential for orthopedic implants.
Highlights
Every year, millions of people suffer from massive bone defects due to various reasons, such as tumors, trauma, and infections, resulting in an increasing clinical demand for bone defect implants (Bhattacharjee et al, 2017; Wang and Yeung, 2017)
Co-cultivation of mesenchymal stem cells with 3D printed Ta-PCL scaffolds 7 days later, Figure 5A showed that the COL I expression of the Ta-PCL scaffold was higher than that of the PCL scaffold alone, indicating that the addition of Ta can promote the secretion of collagen, which may further promote the formation of calcium nodules. 5%Ta-PCL had the highest expression of COL I, followed by 10 and 1%Ta-PCL
We obtained a scaffold with good pore structure and porosity by 3D printing the mixture of PCL and Ta nanoparticles
Summary
Millions of people suffer from massive bone defects due to various reasons, such as tumors, trauma, and infections, resulting in an increasing clinical demand for bone defect implants (Bhattacharjee et al, 2017; Wang and Yeung, 2017). Tantalum and nanotantalum have been proved to have the characteristics of promoting the osteogenic differentiation of mesenchymal stem cells (Mohandas et al, 2014; Wang et al, 2015; Kang et al, 2017). We first used nano-tantalum blended PCL with 3D printing to enhance PCL, and fabricated Ta-PCL doped with different proportions of tantalum nanoparticles (1, 5 and10%wt) to study the effect of the 3D Ta-PCL scaffold on the osteogenic differentiation of mesenchymal stem cells in vitro. Our hypothesis is that Ta nanoparticles can enhance the crystallinity and hydrophilic properties of PCL (Supplementary Figure S3) and further enhance the osteogenic differentiation ability of mesenchymal stem cells. The osteogenic differentiation ability of 3D Ta-PCL was tested by RT-PCR and COL I staining
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