Tannins and Related Compounds in Callus and Root Cultures of Cornus capitata.

Abstract

Callus and adventitious root cultures of Cornus capitata were established and the production of tannins and related compounds was determined in various culture conditions (media, plant growth regulators, light, etc.). Calli produced mainly procyanidins such as (+)-catechin and procyanidin B-3 on Murashige and Skoog (MS) and Woody Plant (WP) media. The maximum content of (+)-catechin (0.1%, as dry weight) was observed in the calli cultured on WP medium supplemented with 0.1mg/l 2, 4-dichlorophenoxyacetic acid (2, 4-D) and 0.1mg/l benzyladenine (BA), or 2.0mg/l naphthaleneacetic acid (NAA) and 0.1mg/l BA in the light. In contrast, adventitious roots cultured in MS liquid medium supplemented with 3.0mg/l indoleacetic acid (IAA) produced a high level of gallotannins such as 1, 2, 3, 6-tetra-O-galloyl-β-D-glucose and 1, 2, 3, 4, 6-penta-O-galloyl-β-D-glucose.