Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs.

Zhi Yin,Jing-Tao Sun,Sanghoon Lee,Chao-Qian Jiang,Yu-Ting Zhang,Zhong-Hua Liu,Jun-Xue Jin,Hong-Di Cui

doi:10.3390/antiox10101594

Abstract

To investigate the effects of tannins (TA) on porcine oocyte in vitro maturation (IVM), different concentrations of TA (0, 1, 10 and 100 μg/mL) were supplemented with a maturation medium and the COCs and subsequent embryonic development were examined. The results showed that 10 µg/mL TA significantly improved the cumulus expansion index (CEI), cumulus-expansion-related genes (PTGS1, PTGS2, PTX-3, TNFAIP6 and HAS2) expression and blastocyst formation rates after parthenogenetic activation (PA), in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT) compared to the control groups, but not oocyte nuclear maturation. Nevertheless, 10 µg/mL TA dramatically enhanced the mRNA expression of oocyte-development-related genes (BMP15, GDF9, CDC2 and CYCLIN B1), GSH, ATP, SOD1, PGC1α, BMP15, GDF9 and CDC2 levels and reduced intracellular ROS level in porcine oocytes. These results indicated that porcine oocyte cytoplasmic maturation was improved by 10 µg/mL TA treatment during IVM. In contrast, a high concentration of TA (100 μg/mL) significantly decreased the CEI and PTGS1, PTGS2, PTX-3 and HAS2 mRNA expressions in cumulus cells, and reduced oocyte nuclear maturation and the total cell numbers/blastocyst. In general, these data showed that 10 μg/mL TA supplementation has beneficial effects on oocyte cytoplasmic maturation and subsequent embryonic development in pigs.

Highlights

Oocyte in vitro maturation (IVM) is one of the most critical steps in in vitro embryo production, which promises to improve the production of genome-modified domestic animals [1]
50 cumulus–oocyte complexes (COCs) were put into a 500 μL IVM culture medium comprising tissue culture medium-199 (TCM-199) supplemented with 0.6 mM cysteine, 0.1 mM sodium pyruvate, 10% porcine follicular fluid (v/v), 10 ng/mL epidermal growth factor (EGF), 10 IU/mL luteinizing hormone (LH), 10 IU/mL follicle-stimulating hormone (FSH) and different concentrations of TA (0, 1, 10 and 100 μg/mL; 403040, Sigma)
TA compared to the control and 100 μg/mL TA

Summary

Introduction

Oocyte in vitro maturation (IVM) is one of the most critical steps in in vitro embryo production, which promises to improve the production of genome-modified domestic animals [1]. The porcine zygote metabolic reserves, the timing of embryo development to blastocyst, amino acid metabolism, zygotic genome activation (ZGA), the genome structure and physiological characteristics are similar to humans [2]. The porcine oocyte is an ideal model to understand the development of female germ cells in humans, and to evaluate food safety and toxicology during embryogenesis. It is thought that TA could protect leaves against insects by deterrence and/or toxicity and induce reactive oxygen species (ROS) development in prostate cancer cells by inhibiting lipid metabolism [3,7].

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Conclusion