Abstract
The disadvantages of metal-coating techniques in high resolution SEM are limitation of resolution due to the thickness of coating, metal decoration of the surface ultrastructures, the lack of uniformity and continuity of coating or specimen damage during coating due to heat generation. In order to overcome these disadvantages and to obtain the better ultrastructural preservation after drying of specimens, the conductive staining methods have been devised.˜4The microwave irradiation(MWI) has recently been used for fixation of biological specimens for light and electron microscopy, immunohistochemistry and for acceleration of the time required for tissue processing and staining. In the present study, MWI can be successfully applied to each step in the tannin- ferrocyanide-OsO4(TA- FeCN-Os) method for high resolution SEM.The TA- FeCNsOs method for high resolution SEM [A] Primary Osmication(Fixation): double fixation with glutaraldehyde-paraformaldehyde and OsO4, TA-FeCN-Os method for TEM,Osmium-DMSO-Osmium, prolonged osmication or osmication after extraction with saponin, glycerol or detergents.
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