Tankyrase-1 mRNA expression in bladder cancer and paired urine sediment: preliminary experience

Stefania Gelmini,Gianluca Giannarini,Mario Pazzagli,Fabrizio Travaglini,Claudio Orlando,Giulio Nicita,Donata Villari,Silvia Quattrone,Alessandro Della Melina,Francesca Malentacchi,Cesare Selli

doi:10.1515/cclm.2007.133

Stefania Gelmini, Gianluca Giannarini + Show 9 more

Open Access

https://doi.org/10.1515/cclm.2007.133

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Abstract

The enzyme tankyrase-1 (TNKS-1), a member of the growing family of poly(ADP-ribose) polymerases (PARPs), was identified as a component of the human telomeric complex. PARPs catalyze the formation of long chains of poly(ADP-ribose) onto protein acceptors using NAD(+) as a substrate. TNKS-1 interacts with the telomeric DNA-binding protein TTAGGG repeat-binding factor 1 (TRF1), which is a negative regulator of telomere length. TNKS-1 is a positive regulator of telomere elongation and its activity appears to be upregulated in some human cancers. We evaluated for the first time TNKS-1 mRNA expression by real time RT-PCR in tumor tissue, paired normal mucosa and urine sediment in patients with transitional cell carcinoma (TCC) of the bladder. Samples were collected from 41 consecutive patients, 20 with non-muscle-invasive (pTa-pT1) and 21 with muscle-invasive (>/=pT2) bladder TCC. Results obtained in urine sediment were compared with those from 40 healthy subjects matched for age and sex. In pTa-pT1 tumor tissues, TNKS-1 mRNA levels were significantly higher than in >/=pT2 patients (p<0.0001). In urine sediment from TCC patients, independent of tumor stage, TNKS-1 mRNA levels were significantly higher than in healthy controls, with maximal levels in >/=pT2 patients. In particular, TNKS-1 mRNA levels in urine were elevated in 31/41 patients with a sensitivity of 81% in >/=pT2 tumors and 65% in pTa-pT1 TCC. Of patients with pTa-pT1 tumors, 11 had a recurrence within 18 months after initial transurethral resection. In these patients, urine levels of TNKS-1 mRNA were higher than in non-relapsing patients (p=0.038). In this preliminary study, TNKS-1 mRNA in urine sediment from patients with bladder TCC correlated with tumor stage, and higher preoperative levels were associated with increased risk of early recurrence.

Highlights

Extreme intense respiratory disorder COVID (SARS-CoV) has caused more than 4012,000 contaminations and >32,000 deaths in New York State alone
The rule behind the Lateral Flow Assay (LFA) is basic: a fluid sample containing the analyte of premium moves without the help of external forces through different zones of polymeric strips, on which particles that can communicate with the analyte are attached [2]
Lateral flow assays can be created to be utilized in a dipstick design or in a housed tape

Summary

INTRODUCTION

Extreme intense respiratory disorder COVID (SARS-CoV) has caused more than 4012,000 contaminations and >32,000 deaths in New York State alone. Because of delay in testing and asymptomatic diseases the genuine number of cases is obscure. Any reports have described the commonness of seroconversion in local area population. Seroconversion, the interaction wherein a patient accumulates antigen-specific antibodies against an epitope, is the initial move towards the advancement of versatile invulnerability against microorganisms. Despite the fact that it's anything but an affirmation of insurance against future diseases, positive seroconversion is a useful proportion of past viral infectivity inside the population. To survey the seroconversion of a local area, immunizer testing with high affectability and particularity that is accessible is important [1]. The rule behind the LFA is basic: a fluid sample (or its concentrate) containing the analyte of premium moves without the help of external forces (capillary action) through different zones of polymeric strips, on which particles that can communicate with the analyte are attached [2]

Types of lateral flow assays

Findings

CONCLUSION