Abstract
The Tandem Scanning Reflected Light Microscope (TSRLM) makes possible the investigation of microscopic structures both at and deep to the surface of intact objects. The present study was undertaken to determine whether the TSRLM would be usable and useful in the investigation of natural and restorative dental materials in vitro and in vivo. It was found that the TSRLM could be used to study the interface between tooth and optically translucent materials in bulk samples, giving high resolution information from only a shallow depth of field but at planes below cut surfaces. This makes it possible to study interfacial regions in three dimensions without the risk of delamination that must accompany the preparation of a microscopic section. This is an important advance in the microscopic assessment of adaptation of biomaterials to hard tissues.
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