Tandem Mass Tag-Based Quantitative Proteomic Analysis of Chicken Bursa of Fabricius Infected With Reticuloendotheliosis Virus.

Dahan Yang,Shujun Zhang,Shimin Zheng,Xiaoping Lv

doi:10.3389/fvets.2021.666512

Abstract

Reticuloendotheliosis virus (REV) is a type C avian retrovirus that causes immunosuppression, dwarf syndrome, and lymphoma in infected hosts. In this study, we used tandem mass tag (TMT) labeling and liquid chromatography–tandem mass spectrometry (LC-MS/MS) to characterize protein alterations in chicken bursa of Fabricius, before and after REV infection at 7, 14, 21, and 28 days. Our data showed that 1,127, 999, 910, and 1,138 differentially expressed proteins were significantly altered at 7, 14, 21, and 28 days after REV infection, respectively. Morphological analysis showed that REV infection reduced in cortical lymphocytes, bursal follicle atrophy, and nuclear damage. Bioinformatics analysis indicated these proteins were mainly involved with immune responses, energy metabolism, cellular processes, biological regulation, metabolic processes, response to stimuli, and multicellular organismal process. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway cluster analysis showed that post-infection, proteins were enriched in the cell cycle, Wnt signaling, antigen processing and presentation, cytokine receptor interaction, adenosine 3′,5′-cyclic monophosphate signaling pathway, and NF-κB signaling. In addition, we observed that peroxiredoxin 4 (PRDX4), peroxiredoxin 6 (PRDX6), glutathione peroxidase 3 (GPX3), catalase (CAT), and peroxidasin (PXDN) were involved in oxidative stress. Some heat shock protein (HSP) family members such as HSPH1, DNAJA4, HSPA8, and HSPA4L also changed significantly after REV infection. These findings help clarify interactions between REV and the host and provides mechanistic insights on REV-induced host immunosuppression.

Highlights

In poultry, reticuloendotheliosis virus (REV) infection leads to immunosuppression, acute reticuloma, short stature syndrome, and lymphoid and other chronic tumorous proliferative diseases [1]
Control group ultrastructure appeared normal under electron microscopy; apoptosis, mitochondrial swelling, and necrosis were observed in the Reticuloendotheliosis virus (REV) infection group
Proteins were differentially expressed at four time points after REV infection

Summary

Introduction

Reticuloendotheliosis virus (REV) infection leads to immunosuppression, acute reticuloma, short stature syndrome, and lymphoid and other chronic tumorous proliferative diseases [1]. REV prevalence is primarily combined with other immunosuppressive viruses such as Marek’s disease virus (MDV) and subgroup J avian leukosis virus, which complicate disease diagnosis and prevention [2, 3]. Proteome in Reticuloendotheliosis Virus Infection of host infection with other pathogenic microorganisms and decreases immune responses to vaccines [4, 5]. The bursa of Fabricius is the main center for B cell development, maturation and differentiation, and REV infection. We studied the proteomics of the bursa of Fabricius after REV infection to understand REV pathogenic mechanisms and different immune responses against REV [6]

Methods

Results

Conclusion