Abstract
11-Dehydrothromboxane B 2 is one of the major enzymatic metabolites of thromboxane B 2 (TXB 2), a biologically inactive product of thromboxane A 2. The short half-life of thromboxane A 2 and ex vivo production of thromboxane B 2 by platelet activation make these prostanoid metabolites inappropriate as indices of systemic thromboxane biosynthesis, whereas 11-dehydro-TXB 2 has been shown to reflect the release of thromboxane A 2 in the human blood circulation. Analysis of 11-dehydro-TXB 2 in plasma and urine was performed by gas chromatography-mass spectrometry-mass spectrometry using the chemically synthesized tetradeuterated compound as an internal standard. The high selectivity of triple-stage quadrupole mass spectrometry (tandem mass spectrometry) considerably facilitates sample purification as compared to single quadrupole mass spectrometric determination. Plasma concentrations in five healthy male volunteers were in the range 0.8–2.5 pg/ml. Urinary excretion of 11-dehydro-TXB 2 was higher than that of 2,3-dinor-TXB 2: 1.2 ± 0.36 μ g 24 h vs 0.53 ± 0.33 μ g 24 h ( n = 5). Thus 11-dehydro-TXB 2 appears at present to be the best index metabolite of systemic TXA 2 activity in plasma as well as in urine.
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