Tandem location of the cellulase genes on the chromosome ofBacillussp. strain N-4

Abstract

A 5.7-kb EcoRI DNA fragment has been isolated from Bacillus sp. strain N-4 chromosome DNA. This fragment contained both the pNK1-encoded cellulase (celB) gene and the pNK2-encoded cellulase (celA) gene which were highly homologous [13]. These results demonstrate the tandem location of these genes on the chromosomal DNA. The homologous sequence, which may play an important role for the gene duplication, were observed 5′ upstream of the celA gene, between the celA and celB genes, and 3′ downstream from the celB gene.