Abstract
The hydroxyl radical-mediated formation of two tandem base lesions within DNA including N-(2-deoxy-β-d-erythro-pentofuranosyl)formylamine-8-oxo-7,8-dihydro-2‘-deoxyguanosine (dβF-8-oxodGuo) and 8-oxodGuo-dβF is reported in this study. A specific enzymatic processing was developed to quantitatively release the tandem lesions as dinucleoside monophosphates from DNA. Then, the resulting hydrolyzed DNA samples were analyzed using liquid chromatography coupled to electrospray ionization tandem mass spectrometry. The simultaneous measurement of the two vicinal lesions was performed in the negative mode using the accurate and sensitive multiple reaction monitoring technique. For this purpose, two characteristic ions arising from the fragmentation of the pseudo-molecular ion [M − H]- were monitored. Both dβF-8-oxodGuo and 8-oxodGuo-dβF damage were found to be generated in γ-irradiated DNA as a significant fraction of •OH radical-induced base damage. Interestingly, 8-oxodGuo-dβF was produced in a much higher yield...
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
