Abstract
We have cloned a region of repetitive DNA from the phytopathogenic fungus, Phytophthora parasitica. The cloned region consists of 17 highly homologous units arranged in tandem. The consensus sequence is 562 bp long and carries the information for a tRNA Asp. All sequence motifs required for efficient RNA polymerase HI transcription are present, and the tRNA derived from the nucleotide sequence is able to form a complete cloverleaf structure with high homology to previously characterized tRNA Asp molecules. The isolated tRNA Asp gene cluster is located at a distance of 20 kb from the TRP1 gene of P. parasitica. It comprises about 0.1 % of the total genomic DNA. Similar clusters were detected in four other Phytophthora species.
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