Abstract
From 101 to 106 TCID50 of herpes simplex viruses types I and II in 0.10 ml volumes can be quantitatively recovered from vaginal tampons in vitro. The distal several centimeters of tampon were extracted in Hanks buffered saline containing fetal calf serum, penicillin, streptomycin and amphotericin B, for end-point titration. Virus could be recovered from 7 brands of tampons.In 5 patients with clinically apparent genital herpes simplex infection, viral cultures were obtained from vulvar vesicles, from direct cervical swabs performed at pelvic examination and from tampons inserted by the patient. Virus was eluted from swabs and tampons and end-point titrations were performed. The cervical viral titers recovered by the 2 methods were similar:Thus, quantitative cervicovaginal cultures can be obtained by tampon culture without pelvic examination.
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