Abstract
Modern high-throughput sequencing technologies are becoming a game changer in many fields of aquatic research and biomonitoring. To unfold their full potential, however, the independent development of approaches has to be streamlined. This discussion must be fuelled by stakeholders and practitioners and, scientific results collaboratively filtered to identify the most promising avenues. Furthermore, aspects such as time, budget, skills and the application context have to be considered, finally communicating good practice strategies to target audiences. Since 2016, the EU COST Action DNAqua-Net is taming the wild west of molecular tools application in aquatic research and biomonitoring. After nucleating available knowledge by the formation of a highly international and transdisciplinary network of scientists, stakeholders, practitioners and enterprises, fields of high methodological diversity were identified. Relevant aspects are currently ground truthed, thereby reducing the plethora of pipelines, parameters and protocols to a subset of good practices or standardisations. To effectively bridge the science-application interface, the very same network is exploited for the dissemination of results (Leese et al. 2018). The internal working group structure of DNAqua-Net is used to provide an overview of existing methodological fields of diversity in DNA-based aquatic biomonitoring: WG1 -DNA Barcode
Highlights
Modern high-throughput sequencing technologies are becoming a game changer in many fields of aquatic research and biomonitoring
Since 2016, the EU COST Action DNAqua-Net is taming the wild west of molecular tools application in aquatic research and biomonitoring
The internal working group structure of DNAqua-Net is used to provide an overview of existing methodological fields of diversity in DNA-based aquatic biomonitoring: WG1 - DNA Barcode References: Different marker systems are targeted for the same organism group
Summary
Modern high-throughput sequencing technologies are becoming a game changer in many fields of aquatic research and biomonitoring. Received: 12 Jun 2019 | Published: 19 Jun 2019 Citation: Weigand A, Bouchez A, Boets P, Bruce K, Ciampor F, Ekrem T, Fontaneto D, Franc A, Hering D, Kahlert M, Keskin E, Mergen P, Pawlowski J, Kueckmann S, Leese F (2019) Taming the Wild West of Molecular Tools Application in Aquatic Research and Biomonitoring. Aspects such as time, budget, skills and the application context have to be considered, communicating good practice strategies to target audiences.
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