Tailoring the photoluminescence of capmatinib towards a novel ultrasensitive spectrofluorimetric and HPLC-DAD monitoring in human serum; investigation of the greenness characteristics

Abstract

This work reports for the first spectrofluorimetric investigation of the INC280, Capmatinib (CAPM). The photophysical characteristics of CAPM especially fluorescence was extensively studied. CAPM has a native green fluorescence at λem = 500 nm with λex = 250 nm, that could be influenced and optimized under various experimental conditions of solvents, pH, temperature and inclusion matrices. Generally, protic polar alcoholic solvents are the best solvents in sensitizing CAPM emission. Furthermore, addition of disodium hydrogen phosphate aqueous solution of pH 6 at 25 °C reveals the highest fluorescence intensity of CAPM. Sodium dodecyl sulfate (SDS) when added to CAPM in optimized concentration results in 81.4 % enhancement in the CAPM fluorescence signal which impacted an improved sensitivity in the fluorimetric assaying of CAPM. HPLC-DAD method was also applied for determination of cited drug. Native spectrofluorimetry and HPLC-DAD of CAPM exhibits wide linear dynamic range from 0.015 to 2.0 µg/ mL and 0.02–100 µg/ mL within limits of detection of 0.0038 and 0.0039 µg/ mL, respectively. Moreover, the two methods are of high accuracy and precision that reveal estimates of 93.16–100.91 % and 96.67–100.42 % for recovery % while the corresponding estimates of RSD% ranges from 0.926 to 2.668 % and 0.505–1.571 %. The two methods were validated with successful applicability in the assaying of spiked CAPM in human serum samples. Greenness characteristics for both spectroflourimetric and HPLC-DAD methods were assessed using AGREE and GAPI approaches.