Abstract
BackgroundCatalase (EC 1.11.1.6) is one of the important industrial enzyme employed in diagnostic and analytical methods in the form of biomarkers and biosensors in addition to their enormous applications in textile, paper, food and pharmaceutical sectors. The present study demonstrates the utility of a newly isolated and adapted strain of genus Geobacillus possessing unique combination of several industrially important extremophilic properties for the hyper production of catalase. The bacterium can grow over a wide range of pH (3–12) and temperature (10–90 °C) with extraordinary capability to produce catalase.ResultsA novel extremophilic strain belonging to genus Geobacillus was exploited for the production of catalase by tailoring its nutritional requirements and process variables. One variable at a time traditional approach followed by computational designing was applied to customize the fermentation process. A simple fermentation media containing only three components namely sucrose (0.55 %, w/v), yeast extract (1.0 %, w/v) and BaCl2 (0.08 %, w/v) was designed for the hyperproduction of catalase. A controlled and optimum air supply caused a tremendous increase in the enzyme production on moving the bioprocess from the flask to bioreactor level. The present paper reports high quantum of catalase production (105,000 IU/mg of cells) in a short fermentation time of 12 h. To the best of our knowledge, there is no report in the literature that matches the performance of the developed protocol for the catalase production. This is the first serious study covering intracellular catalase production from thermophilic genus Geobacillus.ConclusionsAn increase in intracellular catalase production by 214.72 % was achieved in the optimized medium when transferred from the shake flask to the fermenter level. The extraordinary high production of catalase from Geobacillus sp. BSS-7 makes the isolated strain a prospective candidate for bulk catalase production on an industrial scale.
Highlights
Catalase (EC 1.11.1.6) is one of the important industrial enzyme employed in diagnostic and analytical methods in the form of biomarkers and biosensors in addition to their enormous applications in textile, paper, food and pharmaceutical sectors
Balwinder Singh Sooch (BSS)-7 cells separated from the fermented broth and no extracellular catalase activity was found in the supernatant
It was analysed from the growth curve that Geobacillus sp
Summary
Catalase (EC 1.11.1.6) is one of the important industrial enzyme employed in diagnostic and analytical methods in the form of biomarkers and biosensors in addition to their enormous applications in textile, paper, food and pharmaceutical sectors. The present study demonstrates the utility of a newly isolated and adapted strain of genus Geobacillus possessing unique combination of several industrially important extremophilic properties for the hyper production of catalase. Many strategies have been employed for the synthesis of catalase from microorganisms, which include optimization of critical factors, induction by H2O2, mutagenesis and other advanced genetic engineering approaches All of these strategies failed to produce commercially viable catalase from microorganisms under adverse industrial process conditions [6, 7]. The present study was carried out to explore catalase producing potential of an endospore forming newly isolated and adapted extremophilic bacterium Geobacillus sp. The 16S rRNA sequence of this isolate has been submitted to the Genbank (NCBI) under an accession number KJ472212 This piece of research focuses on enhancing the microbial production of catalase by tailoring nutritional and multiple bioprocess parameters at the flask level (Fig. 1). A comparative analysis of scientific literature reveals that the catalase yield in the present developed process is much better than other reported microbial sources like Rhizobium radiobacter (30,420 IU/mg in 20 h) [8], Exiguobacterium oxidotolerans T-2-2T (16,000 IU/mg in 24 h) [9], Thermoascus aurantiacus (5100 IU/mg in 20 days) [10] and Vibrio rumoiensis (4092 IU/mg in 48 h) [11]
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