Abstract
Molecular imprinted titania nanoparticles were developed for selective recognition of purines, for example, guanine and its final oxidation product uric acid. Titania nanoparticles were prepared by hydrolysis of titanium butoxide as precursor in the presence of pattern molecules. The morphology of synthesized nanoparticles is evaluated by SEM images. Recognition characteristics of imprinted titania nanoparticles are studied by exposing them to standard solution of guanine and uric acid, respectively. The resultant change in their concentration is determined by UV/Vis analysis that indicated imprinted titania nanoparticles possess high affinity for print molecules. In both cases, nonimprinted titania is taken as control to observe nonspecific binding interactions. Cross sensitivity studies suggested that imprinted titania is at least five times more selective for binding print molecules than competing analyte thus indicating its potential for bioassay of purines.
Highlights
Molecular imprinting technology (MIT) is getting increasing attention of scientific community owing to its numerous applications [1] in various fields such as catalysis [2], chiral separations [3], chemical sensor design [4], advance drug delivery systems [5], process control [6], and many others
As-prepared molecular imprinted titania nanoparticles were washed with water and methanol solvent system to release print molecules from titania network and remove unwanted reaction species
The supernatant of washed nanoparticles was analyzed by UV/Vis in the range of 200–400 nm to observe the absorbance of released print molecules
Summary
Molecular imprinting technology (MIT) is getting increasing attention of scientific community owing to its numerous applications [1] in various fields such as catalysis [2], chiral separations [3], chemical sensor design [4], advance drug delivery systems [5], process control [6], and many others. MIT is one of the most promising approaches to design synthetic receptor materials as molecular imprinted polymers offer both chemical and geometrical fitting [9] to target molecules. The washed polymer possesses adapted interaction centers complementary to the shape and dimensions of template; it offers the geometrical fitting for target analyte [10]. The major concern in using bulk imprinted polymeric materials was inhomogeneous particles of large size due to mechanical crushing and grindings. This often leads to small recoverable mass of imprinted particles and less efficient bindings. In recent years the focus has been shifted from bulk imprinted polymers to nanosized particles that offer high surface area with promising sensing [13, 14] potential
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