Abstract
Inflammatory bowel disease (IBD), including Crohn’s disease (CD) and ulcerative colitis (UC), is increasingly prevalent and current therapies are not completely effective. Mesenchymal stem cells are emerging as a promising therapeutic option. Here, the effect of local hydrogel application loaded with conditioned medium (CM) from human uterine cervical stem cells (hUCESC-CM) in an experimental acute colitis mice model has been evaluated. Colitis induction was carried out in C57BL/6 mice by dissolving dextran sulfate sodium (DSS) in drinking water for nine days. Ulcers were treated by rectal administration of either mesalazine (as positive control) or a mucoadhesive and thermosensitive hydrogel loaded with hUCESC-CM (H-hUCESC-CM). Body weight changes, colon length, and histopathological analysis were evaluated. In addition, pro-inflammatory TNF-α, IL-6, and IFN-γ mRNA levels were measured by qPCR. Treatment with H-hUCESC-CM inhibited body weight loss and colon shortening and induced a significant decrease in colon mucosa degeneration, as well as TNF-α, IFN-γ, and IL-6 mRNA levels. Results indicate that H-hUCESC-CM effectively alleviated DSS-induced colitis in mice, suggesting that H-hUCESC-CM may represent an attractive cell-free therapy for local treatment of IBD.
Highlights
Inflammatory bowel disease (IBD) represents a group of pathogenic, chronic, and degenerative disorders defined by recurrent inflammation of the gastrointestinal tract.The two major forms are ulcerative colitis (UC) and Crohn’s disease (CD)
Dispersions of Pluronic® copolymers at low temperatures exist in the form of unimers even at high concentration
The addition of PF68 to a dispersion of Pluronic® F127 (PF127) disturbs the formation of PF127 micelles
Summary
Inflammatory bowel disease (IBD) represents a group of pathogenic, chronic, and degenerative disorders defined by recurrent inflammation of the gastrointestinal tract. The two major forms are ulcerative colitis (UC) and Crohn’s disease (CD). It is estimated that as many as 1.4 million Americans and 2.4 million Europeans are suffering from this disease [1]. The incidence and prevalence of IBD have increased dramatically over the last few decades, growing as a public health concern since they can lead to lifethreatening problems [2,3]. IBD is multifactorial and its aetiology is not precisely known. IBD involves an abnormal systemic and mucosal immune response against intraluminal antigens, favoured by microbial factors and alterations of the mucosal barrier, in genetically predisposed individuals [4,5]
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