Taibaiella lutea sp. nov., Isolated from Ubiquitous Weedy Grass.

Abstract

An aerobic, Gram-stain-negative, non-motile and rod-shaped bacterial strain, designated as KVB11T was isolated from the root of plant Setaria viridis near Dongguk University. Phylogenetic analysis indicated that strain KVB11T belonged to the genus Taibaiella of the family Chitinophagaceae. Comparative 16S rRNA gene analysis indicated that the strain KVB11T has closest similarities to Taibaiella smilacinae PTJT-5T (96.6%) and Taibaiella yonginensis THG-SC4T (96.4%). Strain KVB11T grew optimally at 25-28°C, at pH 7.0 and tolerates NaCl upto 1% (w/v) NaCl. Flexirubin-type pigments were present. Phylogenetic and phylogenomic trees based on 16S rRNA gene sequences and genomic sequences demonstrated that strain KVB11T formed a distinct branch with all seven type strains of genus Taibaiella. MK-7 was identified as the predominant isoprenoid quinone. The polar lipid were phosphatidylethanolamine, one unidentified aminophosphoglycolipid, one unidentified aminoglycolipid and two unidentified phosphoglycolipids. C15:0 (28.3%), iso-C15:1 G (28.5%) and iso-C17:0 3-OH (10.5%) were the major fatty acids of the strain KVB11T. The DNA G + C content of the genomic DNA of novel isolate was determined to be 38.9%. The above results clearly confirmed that strain KVB11T represents a novel species of the genus Taibaiella, for which the name Taibaiella lutea sp. nov. is proposed. The type strain is KVB11T (= KACC 19904T = NBRC 113690T).