Abstract
An unlabelled immunoperoxidase method on paraffin sections was used for the diagnosis of pseudorabies (Aujeszky’s disease) in 78 young pigs. The sensitivity and specificity of this new diagnostic method was compared to that of conventional diagnostic methods, namely virus isolation, immunofluorescence and histopathology. It was found that this immunoperoxidase method was specific and sensitive and therefore can be used as an alternative diagnostic method. A limited number of cases also were tested with an indirect immunogold method on paraffin sections. This method was specific but inferior in sensitivity as compared to the immunoperoxidase method. The possibilities, advantages and disadvantages of both immunoperoxidase and immunogold methods are discussed.
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