Abstract
AbstractAn expedient conversion of N‐glycans to the naphthimidazole (NAIM) derivatives is established by the iodine‐promoted oxidative condensation of N‐glycans with 2,3‐naphthalenediamine at room temperature. The NAIM derivatization not only introduces a UV/fluorescence sensitive chromophore but also increases the hydrophobicity of N‐glycans to improve the ionization efficiency in mass spectrometry (MS). Thus, the NAIM derivatives of N‐linked oligosaccharide Man9 and tetra‐antennary N‐glycan NA4 show enhanced sodiated ions in matrix‐assisted laser desorption/ionization time‐of‐flight MS with a detection limit of 50 pmol, whereas the NAIM derivative of sialic acid‐containing tri‐antennary N‐glycan A3 is detected as the protonated ion by linear trap quadrupole Fourier transform MS. Furthermore, the N‐glycans of ovalbumin and fetuin are released and directly converted to the NAIM derivatives for facilitated HPLC and MS analyses. This is the first study of N‐glycan‐NAIM for MS measurement, which is especially valuable in glycan analysis of biological samples containing sensitive subunits.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
