Abstract
Molecular markers are being used to tag genes conferring resistance to bacterial blight of rice. Near-isogenic lines (NILs) for the resistance genes were compared with the susceptible recurrent parent, and polymorphic markers were used for linkage analysis on segregating populations. The genes Xa-1, Xa-2 and Xa-10 were located on restriction fragment length polymorphism (RFLP) linkage map. Xa-1and Xa-2, on chromosome 4, were linked to the RFLP marker XNpb264 at distances 1.97 cM and 2.04 cM, respectively. Xa-10 was located on chromosome 11, 15.7 cM from RFLP marker XNpb78. Random amplified polymorphic DNA (RAPD) markers flanking Xa-1 and Xa-10 were also identified using NILs. One marker was tightly linked to Xa-1. No recombinant was observed between Xa-1 and this marker in 84 F2 plants. Two markers were linked to Xa-10 at distances of 6.3 cM and 19.0 cM, respectively. IR-BB3 and IR-BB4, NILs with Xa-3 and Xa-4, respectively, were analyzed by using RFLP markers. In both lines, introgressed segments containing Xa-3 and Xa-4 were retained at the same end of the RFLP map of chromosome 11.
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