Table_1.xlsx

Abstract

Duck plague virus (DPV) can cause high morbidity and mortality in many waterfowl species within the order Anseriformes. The DPV genome contains 78 open reading frames (ORFs), among which the LORF2, LORF3, LORF4, LORF5 and SORF3 genes are unique genes of avian herpesvirus. In this study, to investigate the role of this unique LORF5 gene in DPV proliferation, we generated a recombinant virus that lack of the LORF5 gene by a two-step red recombination system, which cloned the DPV CHv genome into a bacterial artificial chromosome (DPV CHv-BAC), the proliferation law of LORF5-deleted mutant virus on DEF cells and the effect of LORF5 gene on the life-cycle stages of duck plague virus compared with the parent strain were tested. Our data revealed that the LORF5 gene contributes to the cell-to-cell transmission of duck plague virus, but is not relevant to virus invasion, replication, assembly and release formation. Taken together, this study sheds light on the role of the avian herpesvirus-specific genes LORF5 in DPV proliferation life-cycle. These findings lay the foundation for in-depth functional studies of the LORF5 gene in DPV or other avian herpesviruses.