Table_1.DOCX

Abstract

Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are known as chronic gastrointestinal inflammatory disorders. The present systematic review and meta-analysis was conducted to estimate the prevalence of AIEC isolates and their phylogenetic grouping among IBD patients compared with the controls. A systematic literature search was conducted among published papers by international authors until 30 April 2020 in Web of Science, Scopus, Embase and PubMed databases. The pooled prevalence of AIEC isolates and their phylogenetic grouping among IBD patients as well as in controls was estimated using fixed or random effects models. Furthermore, for estimating the association of colonization by AIEC with IBD odds ratio along with 95% confidence interval was reported. A total of 205 articles retrieved by the initial search of databases, 13 case-control studies met the eligibility criteria for inclusion in the meta-analysis. There were 465 IBD cases (348 CD and 117 UC) and 307 controls. The pooled prevalence of AIEC isolates were 28% (95% CI: 18- 39%), 29% (95% CI: 20- 40%), 13 % (95% CI: 1-30%) and 9% (95% CI: 3-19%), respectively among IBD, CD, UC and control group. Our results revealed that the most frequent AIEC phylogroup in the IBD, CD, and control groups was B2. Fixed-effects meta-analysis showed that colonization of AIEC is significantly associated with IBD (OR: 2.93; 95% CI: 1.90–4.52; P<0.001), and CD (OR: 3.07; 95% CI: 1.99-4.74; P<0.001) but not with UC (OR: 2.29; 95% CI: 0.81- 6.51; P= 0.11). In summary, this meta-analysis revealed that colonization by AIEC is more frequent in IBD and is associated with IBD (CD and UC). Our results suggested that the affecting by IBD in patients colonized with the AIEC pathovar is not randomly, it is in fact a specific disease-related pathovar.