TabDEG: Classifying differentially expressed genes from RNA-seq data based on feature extraction and deep learning framework.

Abstract

Traditional differential expression genes (DEGs) identification models have limitations in small sample size datasets because they require meeting distribution assumptions, otherwise resulting high false positive/negative rates due to sample variation. In contrast, tabular data model based on deep learning (DL) frameworks do not need to consider the data distribution types and sample variation. However, applying DL to RNA-Seq data is still a challenge due to the lack of proper labeling and the small sample size compared to the number of genes. Data augmentation (DA) extracts data features using different methods and procedures, which can significantly increase complementary pseudo-values from limited data without significant additional cost. Based on this, we combine DA and DL framework-based tabular data model, propose a model TabDEG, to predict DEGs and their up-regulation/down-regulation directions from gene expression data obtained from the Cancer Genome Atlas database. Compared to five counterpart methods, TabDEG has high sensitivity and low misclassification rates. Experiment shows that TabDEG is robust and effective in enhancing data features to facilitate classification of high-dimensional small sample size datasets and validates that TabDEG-predicted DEGs are mapped to important gene ontology terms and pathways associated with cancer.