T2 -prepared balanced steady-state free precession (bSSFP) for quantifying whole-blood oxygen saturation at 1.5T.

Abstract

To establish a calibration equation to convert human blood T2 to the full range of oxygen saturation levels (HbO2 ) and physiologic hematocrit (Hct) values using a T2 -prepared balanced steady-state free precession sequence (T2 -SSFP) at 1.5T. Blood drawn from 10 healthy donors (29.1 ± 3.9 years old) was prepared into samples of varying HbO2 and Hct (n = 79), and imaged using T2 -SSFP sequence at 37°C and interrefocusing interval τ180 = 12 ms. The relationship between blood T2 , HbO2 , and Hct was established based on the model R2=R2,plasma+Hct(R2,RBC-R2,plasma)+k·Hct·(1-Hct)·(1-HbO2)2. Measured R2 and HbO2 levels were fit by the model yielding values of R2,plasma, R2,RBC, and k. T2 -SSFP and the established calibration equation were applied to extract HbO2 at the superior sagittal sinus (SSS) in vivo and were compared with susceptometry-based oximetry. Constants derived from the fit were: k = 74.2 [s-1 ], R2,plasma = 1.5 [s-1 ], R2,RBC = 11.6 [s-1 ], the R2 of the fit was 0.95. Average HbO2 at the SSS in seven healthy volunteers was 65% ± 7% and 66% ± 7% via T2 - and susceptometry-based oximetry, respectively. Bland-Altman analysis indicated agreement between the two oximetric methods with no significant bias. The calibration constants presented here should ensure improved accuracy for whole-blood oximetry based on T2 -SSFP at 1.5T. Magn Reson Med 79:1893-1900, 2018. © 2017 International Society for Magnetic Resonance in Medicine.